\nSITIO OFICIAL
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UNIDAD DE INVESTIGACI\u00d3N EN NEURODESARROLLO<\/h2>\n
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La Unidad de Investigaci\u00f3n en Neurodesarrollo tiene como finalidad evaluar, acompa\u00f1ar e intervenir a trav\u00e9s de la neurohabilitaci\u00f3n a reci\u00e9n nacidos expuestos a factores de riesgo para da\u00f1o cerebral en la etapa prenatal y perinatal, tomando ventaja de la alta plasticidad cerebral que existe durante los primeros a\u00f1os de vida.<\/p>\n
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INFORMACI\u00d3N GENERAL<\/h3>\n
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La Unidad de Investigaci\u00f3n en Neurodesarrollo (UIND) \u201cDr. Augusto Fern\u00e1ndez Guardiola\u00bb, adscrita al Instituto de Neurobiolog\u00eda, UNAM Campus Juriquilla realiza evaluaciones diagn\u00f3sticas e interviene a trav\u00e9s de la terapia neurohabilitatoria antes de los 2 meses de vida, disminuyendo o previniendo las secuelas que podr\u00edan provocar los factores de riesgo para da\u00f1o cerebral.
En la UIND, se lleva a cabo un cuidadoso seguimiento de los participantes desde el nacimiento hasta la edad escolar, a trav\u00e9s de evaluaciones neuropedi\u00e1tricas, electroencefalogr\u00e1ficas, de potenciales evocados visuales y auditivos, de imagen por resonancia magn\u00e9tica, sensoriomotoras, de la atenci\u00f3n, nutricionales, del desarrollo del lenguaje y la evaluaci\u00f3n del desarrollo cognitivo y conductual.
Las l\u00edneas de investigaci\u00f3n de la UIND se enfocan en generar conocimiento b\u00e1sico del desarrollo t\u00edpico y at\u00edpico cerebral humano en los primeros a\u00f1os de vida, as\u00ed como en generar conocimiento aplicado en el desarrollo e implementaci\u00f3n de herramientas de diagn\u00f3stico temprano y en la aplicaci\u00f3n de tratamientos cl\u00ednicos innovadores enfocados a disminuir o prevenir las secuelas de los factores de riesgo para da\u00f1o cerebral.<\/p>\n
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BENEFICIOS DEL PROGRAMA DE INVESTIGACI\u00d3N<\/h3>\n
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1. Los ni\u00f1os neurohabilitados tendr\u00e1n una calidad de vida personal, familiar y social mejor, lo cual tendr\u00e1 un impacto profundo en su desarrollo escolar\/laboral y por ende en el \u00e1mbito socioecon\u00f3mico.<\/p>\n
2. El tratamiento preventivo temprano es mucho m\u00e1s eficiente y menos costoso que el tratamiento tard\u00edo de las discapacidades.<\/p>\n
3. La consolidaci\u00f3n de un grupo multidisciplinario enfocado al desarrollo de programas eficientes que ayuden a disminuir la incidencia de individuos discapacitados, mediante la reducci\u00f3n de las secuelas provocadas por da\u00f1os cerebrales, a trav\u00e9s de una intervenci\u00f3n temprana o preventiva.<\/p>\n
4. Desarrollar investigaci\u00f3n y nuevo conocimiento en el \u00e1rea de plasticidad cerebral, as\u00ed como en nuevos m\u00e9todos de prevenci\u00f3n, diagn\u00f3stico y tratamiento de lesiones cerebrales.<\/p>\n
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UNIDAD DE AN\u00c1LISIS CONDUCTUAL<\/h2>\n
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UNAM, CAMPUS JURIQUILLA<\/strong>
Blvd. Juriquilla 3001, Juriquilla
Quer\u00e9taro, M\u00e9xico, 76230<\/p>\nResponsable<\/strong>
Dra. Deisy Gasca Mart\u00ednez
gasca@inb.unam.mx<\/p>\n[\/et_pb_text][et_pb_text disabled_on=\u00bbon|on|on\u00bb _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_font_size=\u00bb12px\u00bb text_line_height=\u00bb1.2em\u00bb background_color=\u00bb#ffffff\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbleft\u00bb custom_margin=\u00bb59px|0px|13px|0px|false|false\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb25px|0px|13px|0px|false|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb custom_padding=\u00bb4px|7px|4px|10px|true|false\u00bb custom_padding_last_edited=\u00bboff|desktop\u00bb link_option_url=\u00bbhttp:\/\/www.inb.unam.mx\/wp-content\/uploads\/2019\/11\/Reglamento_de_la_Unidad_de_Analisis_Conductual.pdf\u00bb link_option_url_new_window=\u00bbon\u00bb border_width_all=\u00bb1px\u00bb border_color_all=\u00bb#aebac1″ border_width_right=\u00bb0px\u00bb border_width_left=\u00bb6px\u00bb border_color_left=\u00bb#425059″ box_shadow_style=\u00bbpreset1″ box_shadow_vertical=\u00bb0px\u00bb box_shadow_blur=\u00bb0px\u00bb disabled=\u00bbon\u00bb locked=\u00bboff\u00bb border_width_left__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon\u00bb custom_padding__hover_enabled=\u00bbon\u00bb custom_padding__hover=\u00bb|32px||\u00bb custom_margin__hover=\u00bb|||-8px\u00bb box_shadow_blur__hover=\u00bb40px\u00bb border_color_left__hover_enabled=\u00bbon|hover\u00bb border_color_left__hover=\u00bb#FF6600″ border_width_left__hover=\u00bb8px\u00bb]<\/p>\n
REGLAMENTO INTERNO DE LA UNIDAD<\/strong><\/p>\n[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_font_size=\u00bb12px\u00bb text_line_height=\u00bb1.2em\u00bb background_color=\u00bb#ffffff\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbleft\u00bb custom_margin=\u00bb30px|0px|13px|0px|false|false\u00bb custom_margin_last_edited=\u00bboff|desktop\u00bb custom_padding=\u00bb4px|7px|4px|10px|true|false\u00bb custom_padding_last_edited=\u00bboff|desktop\u00bb link_option_url=\u00bbhttps:\/\/servicios.inb.unam.mx\/calendarios\/uac\/uac_calendars.html\u00bb link_option_url_new_window=\u00bbon\u00bb border_width_all=\u00bb1px\u00bb border_color_all=\u00bb#aebac1″ border_width_right=\u00bb0px\u00bb border_width_left=\u00bb6px\u00bb border_color_left=\u00bb#FF6600″ box_shadow_style=\u00bbpreset1″ box_shadow_vertical=\u00bb0px\u00bb box_shadow_blur=\u00bb0px\u00bb locked=\u00bboff\u00bb border_width_left__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon\u00bb custom_padding__hover_enabled=\u00bbon\u00bb custom_padding__hover=\u00bb|32px||\u00bb custom_margin__hover=\u00bb|||-8px\u00bb box_shadow_blur__hover=\u00bb40px\u00bb border_color_left__hover_enabled=\u00bbon|hover\u00bb border_color_left__hover=\u00bb#425059″ border_width_left__hover=\u00bb8px\u00bb]<\/p>\n
AGENDAS Y RESERVACIONES<\/strong><\/p>\n[\/et_pb_text][et_pb_button button_url=\u00bb#unidadesdeapoyo\u00bb button_text=\u00bbUNIDADES DE APOYO A LA INVESTIGACION\u00bb button_alignment=\u00bbleft\u00bb _builder_version=\u00bb4.4.0″ custom_button=\u00bbon\u00bb button_text_size=\u00bb15px\u00bb button_text_color=\u00bb#ffffff\u00bb button_bg_color=\u00bb#8794A3″ button_border_width=\u00bb0px\u00bb button_border_color=\u00bbrgba(0,0,0,0)\u00bb button_border_radius=\u00bb0px\u00bb button_letter_spacing=\u00bb1px\u00bb button_font=\u00bbOswald||||||||\u00bb button_icon=\u00bb%%179%%\u00bb button_icon_color=\u00bb#ffffff\u00bb button_icon_placement=\u00bbleft\u00bb button_on_hover=\u00bboff\u00bb custom_margin=\u00bb25px|||||\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb25px||25px||false|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb button_text_size_tablet=\u00bb\u00bb button_text_size_phone=\u00bb12px\u00bb 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UNIDAD DE AN\u00c1LISIS CONDUCTUAL<\/h2>\n
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La\u00a0Unidad de An\u00e1lisis Conductual tiene la finalidad\u00a0de proveer asesor\u00eda, espacio y equipo especializado a estudiantes, t\u00e9cnicos acad\u00e9micos e investigadores que lo requieran. Tiene tambi\u00e9n como objetivo la interacci\u00f3n entre investigadores con experiencia en el an\u00e1lisis de la conducta y otros con menor experiencia en ese rubro, por lo que promueve la colaboraci\u00f3n y culminaci\u00f3n de proyectos multidisciplinarios.<\/p>\n
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SITUACI\u00d3N ACTUAL<\/h3>\n
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La Unidad de An\u00e1lisis Conductual (UnAC) ofrece un espacio equipado para realizar tareas conductuales en ratas y ratones. Proporciona asesor\u00eda t\u00e9cnica, tecnolog\u00eda de seguimiento de video y software especializado. El equipamiento cient\u00edfico permite una bater\u00eda completa de ensayos en los que se eval\u00faa el aprendizaje y la memoria, la funci\u00f3n motora, la ansiedad y la depresi\u00f3n, as\u00ed como el registro de calorimetr\u00eda indirecta.<\/p>\n
Las actividades de la UnAC fomentan la interacci\u00f3n entre investigadores con experiencia en el an\u00e1lisis de la conducta y otros con menor experiencia, por lo que promueve las colaboraciones de proyectos multidisciplinarios.<\/p>\n
El espacio f\u00edsico que ocupa la UnAC consta de diversas salas de an\u00e1lisis y alojamiento temporal y tiene las caracter\u00edsticas esenciales para garantizar los servicios y la \u00f3ptima operaci\u00f3n de los equipos. Los espacios de an\u00e1lisis conductual son sonoamortiguados, tienen iluminaci\u00f3n blanca y roja, cuentan con temporizadores de iluminaci\u00f3n y un sistema de ventilaci\u00f3n y control de temperatura ambiental con especificaciones para Bioterio basados en la Norma Mexicana vigente.<\/p>\n
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UNIDAD DE MICROSCOP\u00cdA<\/h2>\n
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UNAM, CAMPUS JURIQUILLA<\/strong>
Blvd. Juriquilla 3001, Juriquilla
Quer\u00e9taro, M\u00e9xico, 76230<\/p>\nResponsable<\/strong>
Elsa Nydia Hernandez R\u00edos
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Microscop\u00eda Electr\u00f3nica de Transmisi\u00f3n<\/h3>\n
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Microscop\u00eda Confocal<\/h3>\n
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Microscop\u00eda de S\u00faperresoluci\u00f3n<\/h3>\n
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Microscop\u00eda \u00d3ptica y de Fluorescencia<\/h3>\n
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Histolog\u00eda<\/h3>\n
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Cursos disponibles<\/h3>\n
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Calendario<\/h3>\n
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UNIDAD DE MICROSCOP\u00cdA<\/h2>\n
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Realizar estudios y an\u00e1lisis de muestras biol\u00f3gicas y de materiales utilizando diversas t\u00e9cnicas de microscop\u00eda.<\/p>\n
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INFORMACI\u00d3N GENERAL<\/h3>\n
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La Unidad de Microscop\u00eda del Instituto de Neurobiolog\u00eda de la UNAM tiene como objetivo
apoyar las labores de investigaci\u00f3n cient\u00edfica y acad\u00e9mica, en el campo de la microscop\u00eda
fot\u00f3nica, confocal, multifot\u00f3nica, de super-resoluci\u00f3n y electr\u00f3nica de transmisi\u00f3n.
Se tienen microscopios \u00f3pticos fot\u00f3nicos compuestos de fluorescencia, microscopios
confocales (uno de ellos equipado con un laser multifot\u00f3nico) y un microscopio electr\u00f3nico de
transmisi\u00f3n. La Unidad de Microscop\u00eda tambi\u00e9n dispone de equipo semiautomatizado para
procesamiento (fijaci\u00f3n, deshidrataci\u00f3n e infiltraci\u00f3n) corte y tinci\u00f3n de muestras biol\u00f3gicas
usando parafina y resinas ep\u00f3xicas.<\/p>\n
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Microscop\u00eda Electr\u00f3nica de Transmisi\u00f3n<\/h3>\n
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UNAM, Campus Juriquilla<\/strong>
Blvd. Juriquilla 3001, Juriquilla
Quer\u00e9taro, M\u00e9xico, 76230
Cd. de Quer\u00e9taro:238-1033
Otras Ciudades: (442)238-1033<\/p>\nMayores Informes<\/strong>
Ma. Lourdes Palma Tirado
palmat@unam.mx<\/p>\n[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_font_size=\u00bb12px\u00bb text_line_height=\u00bb2em\u00bb background_color=\u00bb#ffffff\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbleft\u00bb custom_margin=\u00bb98px|0px|13px|0px|false|false\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb25px|0px|25px|0px|true|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb custom_padding=\u00bb4px|7px|4px|10px|true|false\u00bb custom_padding_last_edited=\u00bboff|desktop\u00bb link_option_url=\u00bbhttp:\/\/www.inb.unam.mx\/wp-content\/uploads\/2022\/05\/SolSerCert2022.pdf\u00bb link_option_url_new_window=\u00bbon\u00bb border_width_all=\u00bb1px\u00bb border_color_all=\u00bb#aebac1″ border_width_right=\u00bb0px\u00bb border_width_left=\u00bb6px\u00bb border_color_left=\u00bb#425059″ box_shadow_style=\u00bbpreset1″ box_shadow_vertical=\u00bb0px\u00bb box_shadow_blur=\u00bb0px\u00bb locked=\u00bboff\u00bb border_width_left__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon\u00bb custom_padding__hover_enabled=\u00bbon\u00bb custom_padding__hover=\u00bb|32px||\u00bb custom_margin__hover=\u00bb|||-8px\u00bb box_shadow_blur__hover=\u00bb40px\u00bb border_color_left__hover_enabled=\u00bbon|hover\u00bb border_color_left__hover=\u00bb#FF6600″ border_width_left__hover=\u00bb8px\u00bb]<\/p>\n
FORMATO DE SOLICITUD<\/strong><\/p>\n[\/et_pb_text][et_pb_button button_url=\u00bb#unidadesdemicroscopia\u00bb button_text=\u00bbUNIDAD DE MICROSCOP\u00cdA\u00bb button_alignment=\u00bbleft\u00bb _builder_version=\u00bb4.4.0″ custom_button=\u00bbon\u00bb button_text_size=\u00bb15px\u00bb button_text_color=\u00bb#ffffff\u00bb button_bg_color=\u00bb#8794A3″ button_border_width=\u00bb0px\u00bb button_border_color=\u00bbrgba(0,0,0,0)\u00bb button_border_radius=\u00bb0px\u00bb button_letter_spacing=\u00bb1px\u00bb button_font=\u00bbOswald||||||||\u00bb button_icon=\u00bb%%179%%\u00bb button_icon_color=\u00bb#ffffff\u00bb button_icon_placement=\u00bbleft\u00bb button_on_hover=\u00bboff\u00bb custom_margin=\u00bb64px|||||\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb25px||||false|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb button_text_size_tablet=\u00bb\u00bb button_text_size_phone=\u00bb12px\u00bb button_text_size_last_edited=\u00bbon|phone\u00bb 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Microscop\u00eda Electr\u00f3nica de Transmisi\u00f3n<\/h3>\n
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La Microscop\u00eda Electr\u00f3nica de Transmisi\u00f3n ha contribuido significativamente al conocimiento de la c\u00e9lula y su funci\u00f3n. La utilizaci\u00f3n de un fino haz de electrones acelerados a una gran velocidad como fuente de iluminaci\u00f3n, confieren al microscopio electr\u00f3nico una alta resoluci\u00f3n, lo que le convierte en una herramienta indispensable en muchos campos de la biolog\u00eda y la medicina, cuando se trata de estudiar la ultraestructura de c\u00e9lulas y tejidos.<\/p>\n
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PROCESAMIENTO DE MUESTRAS PARA MICROSCOP\u00cdA ELECTR\u00d3NICA DE TRANSMISI\u00d3N<\/h3>\n
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Para el estudio de la ultraestructura celular y de materiales, se ofrece el servicio de fijaci\u00f3n de tejidos, deshidrataci\u00f3n, corte y montaje en rejillas, contraste (incluyendo inmunocitoqu\u00edmica) y observaci\u00f3n en el microscopio electr\u00f3nico de transmisi\u00f3n. Adem\u00e1s se ofrece el servicio de obtenci\u00f3n de navajas de vidrio para ultramicrotom\u00eda.<\/p>\n
Para el corte de muestras la Unidad de Microscop\u00eda cuenta con un Ultramicrotomo de la marca RMC modelo MT-x y una m\u00e1quina para hacer cuchillas de la misma marca.
El microscopio electr\u00f3nico de transmisi\u00f3n con el que cuenta la Unidad es de la marca JEOL, modelo JEM-1010, el cual puede trabajar en un rango de 60-80 Kv con resoluci\u00f3n de 0.25 nm aproximadamente. El microscopio electr\u00f3nico tiene acoplada una c\u00e1mara Gatan Bioscan de 1Kx1K que utiliza el programa Digital Micrograph 3.1 para adquisici\u00f3n de im\u00e1genes.<\/p>\n
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Microscop\u00eda CONFOCAL<\/h3>\n
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UNAM, Campus Juriquilla<\/strong>
Blvd. Juriquilla 3001, Juriquilla
Quer\u00e9taro, M\u00e9xico, 76230
Cd. de Quer\u00e9taro:238-1033
Otras Ciudades: (442)238-1033<\/p>\nMayores Informes<\/strong>
Elsa Nydia Hernandez R\u00edos
hrios@unam.mx<\/p>\n[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_font_size=\u00bb12px\u00bb text_line_height=\u00bb1.2em\u00bb background_color=\u00bb#ffffff\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbleft\u00bb custom_margin=\u00bb98px|0px|13px|0px|false|false\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb25px|0px|13px|0px|false|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb custom_padding=\u00bb4px|7px|4px|10px|true|false\u00bb custom_padding_last_edited=\u00bboff|desktop\u00bb link_option_url=\u00bbhttp:\/\/www.inb.unam.mx\/wp-content\/uploads\/2019\/11\/protocolo_muestras_confocal.pdf\u00bb link_option_url_new_window=\u00bbon\u00bb border_width_all=\u00bb1px\u00bb border_color_all=\u00bb#aebac1″ border_width_right=\u00bb0px\u00bb border_width_left=\u00bb6px\u00bb border_color_left=\u00bb#425059″ box_shadow_style=\u00bbpreset1″ box_shadow_vertical=\u00bb0px\u00bb box_shadow_blur=\u00bb0px\u00bb locked=\u00bboff\u00bb border_width_left__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon\u00bb custom_padding__hover_enabled=\u00bbon\u00bb custom_padding__hover=\u00bb|32px||\u00bb custom_margin__hover=\u00bb|||-8px\u00bb box_shadow_blur__hover=\u00bb40px\u00bb border_color_left__hover_enabled=\u00bbon|hover\u00bb border_color_left__hover=\u00bb#FF6600″ border_width_left__hover=\u00bb8px\u00bb]<\/p>\n
PROTOCOLO PARA LA EVALUACI\u00d3N DE MUESTRAS<\/strong><\/p>\n[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_font_size=\u00bb12px\u00bb text_line_height=\u00bb2em\u00bb background_color=\u00bb#ffffff\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbleft\u00bb custom_margin=\u00bb19px|0px|13px|0px|false|false\u00bb custom_margin_last_edited=\u00bboff|desktop\u00bb custom_padding=\u00bb4px|7px|4px|10px|true|false\u00bb custom_padding_last_edited=\u00bboff|desktop\u00bb link_option_url=\u00bbhttp:\/\/www.inb.unam.mx\/wp-content\/uploads\/2022\/05\/Solicitud-ServicioMC-2022-1.pdf\u00bb link_option_url_new_window=\u00bbon\u00bb border_width_all=\u00bb1px\u00bb border_color_all=\u00bb#aebac1″ border_width_right=\u00bb0px\u00bb border_width_left=\u00bb6px\u00bb border_color_left=\u00bb#425059″ box_shadow_style=\u00bbpreset1″ box_shadow_vertical=\u00bb0px\u00bb box_shadow_blur=\u00bb0px\u00bb locked=\u00bboff\u00bb border_width_left__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon\u00bb custom_padding__hover_enabled=\u00bbon\u00bb custom_padding__hover=\u00bb|32px||\u00bb custom_margin__hover=\u00bb|||-8px\u00bb box_shadow_blur__hover=\u00bb40px\u00bb border_color_left__hover_enabled=\u00bbon|hover\u00bb border_color_left__hover=\u00bb#FF6600″ border_width_left__hover=\u00bb8px\u00bb]<\/p>\n
FORMATO DE SOLICITUD<\/strong><\/p>\n[\/et_pb_text][et_pb_button button_url=\u00bb#unidadesdemicroscopia\u00bb button_text=\u00bbUNIDAD DE MICROSCOP\u00cdA\u00bb button_alignment=\u00bbleft\u00bb _builder_version=\u00bb4.4.0″ custom_button=\u00bbon\u00bb button_text_size=\u00bb15px\u00bb button_text_color=\u00bb#ffffff\u00bb button_bg_color=\u00bb#8794A3″ button_border_width=\u00bb0px\u00bb button_border_color=\u00bbrgba(0,0,0,0)\u00bb button_border_radius=\u00bb0px\u00bb button_letter_spacing=\u00bb1px\u00bb button_font=\u00bbOswald||||||||\u00bb button_icon=\u00bb%%179%%\u00bb button_icon_color=\u00bb#ffffff\u00bb button_icon_placement=\u00bbleft\u00bb button_on_hover=\u00bboff\u00bb custom_margin=\u00bb64px|||||\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb25px||||false|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb button_text_size_tablet=\u00bb\u00bb button_text_size_phone=\u00bb12px\u00bb button_text_size_last_edited=\u00bbon|phone\u00bb 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Microscop\u00eda CONFOCAL<\/h3>\n
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El microscopio l\u00e1ser confocal es un microscopio \u00f3ptico que incluye como fuente de luz un l\u00e1ser y un sistema electr\u00f3nico que ayuda a la captura de im\u00e1genes. El principio de la microscopia confocal se basa en eliminar la luz reflejada o fluorescente procedente de los planos fuera de foco, donde se ilumina una peque\u00f1a zona de la muestra y se toma el haz luminoso que proviene del plano focal, elimin\u00e1ndose los haces procedentes de los planos inferiores y superiores (Boyde, 1988).<\/p>\n
Microscopio confocal Zeiss LSM 780 DUO<\/strong><\/p>\nEl uso de este equipo es poder implementar t\u00e9cnicas sofisticadas de imagenolog\u00eda confocal multifot\u00f3nica en el animal en vivo y la visualizaci\u00f3n de eventos celulares altamente din\u00e1micos.
El cabezal LSM 780 provee al sistema confocal la capacidad de detectar pr\u00e1cticamente todos los fluoroforos que existen actualmente en el mercado, cuenta con un l\u00e1ser Arg\u00f3n Multil\u00ednea\u00a0 (emisi\u00f3n 458-488-514nm), l\u00e1ser DPSS emisi\u00f3n en 561nm y l\u00e1ser HeNe emisi\u00f3n 633nm, mientras que el cabezal LIVE tiene dos l\u00e1ser de diodo para 489nm y 532nm. Adem\u00e1s de estar equipado con estos dos cabezales, cuenta con dos m\u00f3dulos NDD (non-descanned detector) para la generaci\u00f3n de arm\u00f3nicos secundarios.<\/p>\n
Este sistema est\u00e1 compuesto por un microscopio vertical AxioImager Z2 con objetivos de 10x, 25x (multi-inmersi\u00f3n) 40x, 63x (ambos de inmersi\u00f3n en aceite), platina motorizada y software Zen by Zeiss.<\/p>\n
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INFRAESTRUCTURA<\/h3>\n
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Microscopio confocal Zeiss LSM 780 DUO<\/strong><\/p>\nEl uso de este equipo es poder implementar t\u00e9cnicas sofisticadas de imagenolog\u00eda confocal multifot\u00f3nica en el animal en vivo y la visualizaci\u00f3n de eventos celulares altamente din\u00e1micos.
El cabezal LSM 780 provee al sistema confocal la capacidad de detectar pr\u00e1cticamente todos los fluoroforos que existen actualmente en el mercado, cuenta con un l\u00e1ser Arg\u00f3n Multil\u00ednea\u00a0 (emisi\u00f3n 458-488-514nm), l\u00e1ser DPSS emisi\u00f3n en 561nm y l\u00e1ser HeNe emisi\u00f3n 633nm, mientras que el cabezal LIVE tiene dos l\u00e1ser de diodo para 489nm y 532nm. Adem\u00e1s de estar equipado con estos dos cabezales, cuenta con dos m\u00f3dulos NDD (non-descanned detector) para la generaci\u00f3n de arm\u00f3nicos secundarios.<\/p>\n
Este sistema est\u00e1 compuesto por un microscopio vertical AxioImager Z2 con objetivos de 10x, 25x (multi-inmersi\u00f3n) 40x, 63x (ambos de inmersi\u00f3n en aceite), platina motorizada y software Zen by Zeiss.<\/p>\n
Microscopio Confocal Zeiss Axiovert 200 LSM 510 Meta \u2013 Multif\u00f3tonico<\/strong><\/p>\nSistema donde se realizan capturas de im\u00e1genes en el plano Z para obtener reconstrucciones tridimensionales, proyecci\u00f3n de las secciones recopiladas, medici\u00f3n en tres dimensiones, capturas en el \u00e1rea de inter\u00e9s (ROI\u2019s) y multifluorescencia en canales adjuntos, lo que permite visualizar dos o hasta 8 canales simult\u00e1neamente con el m\u00f3dulo META.<\/p>\n
Microscopio invertido Axiovert 200 con objetivos en 5x,20x, 25x (multi-inmersi\u00f3n agua desionizada, aceite \u00f3 glicerol) 40x, 63x y 100x (todos de inmersi\u00f3n en aceite). Cuenta con un l\u00e1ser Arg\u00f3n Multil\u00ednea\u00a0 (emisi\u00f3n 454-477-488-514nm), l\u00e1ser DPSS emisi\u00f3n en 561nm y l\u00e1ser HeNe emisi\u00f3n 633nm. Adem\u00e1s tiene adaptado el sistema de incubaci\u00f3n para experimentos en vivo, platina caliente, control de temperatura y C02, software LSM ver. 4.2 by Zeiss.<\/p>\n
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Microscop\u00eda DE SUPERRESOLUCI\u00d3N<\/h3>\n
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UNAM, Campus Juriquilla<\/strong>
Blvd. Juriquilla 3001, Juriquilla
Quer\u00e9taro, M\u00e9xico, 76230
Cd. de Quer\u00e9taro:238-1033
Otras Ciudades: (442)238-1033<\/p>\nMayores Informes<\/strong>
Elsa Nydia Hernandez R\u00edos
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Microscop\u00eda DE SUPERRESOLUCI\u00d3N<\/h3>\n
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Microscop\u00eda de S\u00faper Resoluci\u00f3n ELYRA \u2013 PS.1<\/strong>
El sistema ELYRA permite observar la ultraestructura del objeto de inter\u00e9s, contar mol\u00e9culas para cuantificar sus resultados y ver la distribuci\u00f3n de las mol\u00e9culas en un contexto estructural.<\/p>\nPermite observar y resolver objetos en el microscopio a un orden de magnitud (10x) mayor a lo que se puede hacer normalmente en microscopia fot\u00f3nica (de luz), as\u00ed como captar estructuras en alta resoluci\u00f3n de una c\u00e9lula completa en 3D.<\/p>\n
Microscopio invertido Axiovert 200 marca Zeiss cuenta con objetivos de 10x, 25x (multi-inmersi\u00f3n) 63x y 100x (todos de inmersi\u00f3n en aceite), platina motorizada, inserto para portaobjetos, fluoro-dish y dos l\u00e1ser para 405nm y 489 nm. Dicho sistema consta 2 c\u00e1maras de EMCCD con tecnolog\u00eda SR-SIM (superresolution structures ilumination microscopy) tiene la opci\u00f3n de adquirir im\u00e1genes en Z para tener datos en 3D, as\u00ed como manipular la muestra de manera cuidadosa que permanecer\u00e1 estable para poder observarla durante periodos largos.<\/p>\n
M\u00f3dulo 3D-PALM (superresolution photoactivated localization microscopy)<\/strong>\u00a0es una t\u00e9cnica utilizada para la expresi\u00f3n end\u00f3gena de prote\u00ednas fluorescentes foto activadas, donde se puede observar mol\u00e9culas individuales o la interacci\u00f3n entre ellas, incluso las mol\u00e9culas cercanas a la membrana celular plasm\u00e1tica, con una resoluci\u00f3n m\u00e1xima de 20nm.<\/p>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00bb1_4,3_4″ use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb1″ make_equal=\u00bbon\u00bb module_id=\u00bbfluorescencia\u00bb _builder_version=\u00bb4.4.0″ background_color=\u00bb#ffffff\u00bb background_image=\u00bbhttp:\/\/www.inb.unam.mx\/wp-content\/uploads\/2019\/09\/background-inb-4.png\u00bb width=\u00bb90%\u00bb max_width=\u00bb90%\u00bb module_alignment=\u00bbcenter\u00bb custom_margin=\u00bb||0px||false|false\u00bb custom_padding=\u00bb||70px|||\u00bb border_width_bottom=\u00bb1px\u00bb 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MICROSCOP\u00cdA \u00d3PTICA Y DE FLUORESCENCIA<\/h3>\n
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UNAM, Campus Juriquilla<\/strong>
Blvd. Juriquilla 3001, Juriquilla
Quer\u00e9taro, M\u00e9xico, 76230
Cd. de Quer\u00e9taro:238-1033
Otras Ciudades: (442)238-1033<\/p>\nMayores Informes<\/strong>
Elsa Nydia Hernandez R\u00edos
hrios@unam.mx<\/p>\n[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_font_size=\u00bb12px\u00bb text_line_height=\u00bb1.2em\u00bb background_color=\u00bb#ffffff\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbleft\u00bb custom_margin=\u00bb72px|0px|13px|0px|false|false\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb25px|0px|25px|0px|true|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb custom_padding=\u00bb4px|7px|4px|10px|true|false\u00bb custom_padding_last_edited=\u00bboff|desktop\u00bb link_option_url=\u00bbhttp:\/\/www.inb.unam.mx\/wp-content\/uploads\/2021\/02\/Solicitud-de-Servicio-MOFSR.pdf\u00bb link_option_url_new_window=\u00bbon\u00bb border_width_all=\u00bb1px\u00bb border_color_all=\u00bb#aebac1″ border_width_right=\u00bb0px\u00bb border_width_left=\u00bb6px\u00bb border_color_left=\u00bb#425059″ box_shadow_style=\u00bbpreset1″ box_shadow_vertical=\u00bb0px\u00bb box_shadow_blur=\u00bb0px\u00bb locked=\u00bboff\u00bb border_width_left__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon\u00bb custom_padding__hover_enabled=\u00bbon\u00bb custom_padding__hover=\u00bb|32px||\u00bb custom_margin__hover=\u00bb|||-8px\u00bb box_shadow_blur__hover=\u00bb40px\u00bb border_color_left__hover_enabled=\u00bbon|hover\u00bb border_color_left__hover=\u00bb#FF6600″ border_width_left__hover=\u00bb8px\u00bb]<\/p>\n
FORMATO DE SOLICITUD<\/strong><\/p>\n[\/et_pb_text][et_pb_button button_url=\u00bb#unidadesdemicroscopia\u00bb button_text=\u00bbUNIDAD DE MICROSCOP\u00cdA\u00bb button_alignment=\u00bbleft\u00bb _builder_version=\u00bb4.4.0″ custom_button=\u00bbon\u00bb button_text_size=\u00bb15px\u00bb button_text_color=\u00bb#ffffff\u00bb button_bg_color=\u00bb#8794A3″ button_border_width=\u00bb0px\u00bb button_border_color=\u00bbrgba(0,0,0,0)\u00bb button_border_radius=\u00bb0px\u00bb button_letter_spacing=\u00bb1px\u00bb button_font=\u00bbOswald||||||||\u00bb button_icon=\u00bb%%179%%\u00bb button_icon_color=\u00bb#ffffff\u00bb button_icon_placement=\u00bbleft\u00bb button_on_hover=\u00bboff\u00bb custom_margin=\u00bb64px|||||\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb0px||||false|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb button_text_size_tablet=\u00bb\u00bb button_text_size_phone=\u00bb12px\u00bb button_text_size_last_edited=\u00bbon|phone\u00bb 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MICROSCOP\u00cdA \u00d3PTICA Y DE FLUORESCENCIA<\/h3>\n
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MICROSCOPIO \u00d3PTICO \u2013 LEICA<\/strong>
Microscopio vertical de luz transmitida cuenta con una c\u00e1mara integrada ICC50 HD a color con ranura para SDcard, cuenta con objetivos de 4x,10x, 40x y 100x de inmersi\u00f3n en aceite, tiene acoplado una computadora con sistema operativo Win 7 y software para obtener im\u00e1genes LAS EZ ver 3.0 by Leica.<\/p>\nMICROSCOPIO DE CONTRASTE DE FASES Y FLUORESCENCIA \u2013 NIKON<\/strong>
Microscopio vertical modelo Eclipse E-600 cuenta con objetivos de 4x,10x, 20x, 40x, 100x (inmersi\u00f3n en aceite), tiene adaptado una c\u00e1mara modelo CoolSnap de alta velocidad blanco y negro. Con este microscopio se pueden observar las t\u00e9cnicas de campo claro, campo oscuro, contraste de fases y fluorescencia. Adem\u00e1s se pueden obtener im\u00e1genes panor\u00e1micas de cortes histol\u00f3gicos gracias al condensador y los objetivos 1x y 2x. Para la t\u00e9cnica de fluorescencia el microscopio posee una l\u00e1mpara HB-1010 con filtros para observar DAPI (405nm) GFP (488nm) Rhodamine (565nm). Para la obtenci\u00f3n de im\u00e1genes se utilizan el software IPLab para Windows.<\/p>\nMICROSCOPIO PARA FLUORESCENCIA APOTOME \u2013 ZEISS<\/strong>
Microscopio AxioImager vertical con c\u00e1mara de alta velocidad AxioCam, platina motorizada y cabezal Apotome, el cual puede obtener im\u00e1genes en el plano Z logrando profundidad al momento de reconstruir las im\u00e1genes. Adem\u00e1s, cuenta con el m\u00f3dulo MosaiX donde se capturan im\u00e1genes en mosaico, con un m\u00e1ximo de1000 filas por 1000 columnas. Cuenta con objetivos de10x, 25x (multi-inmersi\u00f3n) 40x y 63x de inmersi\u00f3n en aceite, filtros para fluorescencia en DAPI (405nm) \/ GFP (emision 488nm) \/ Rhodamine (565nm) \/ Alexa 594 (600nm). 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HISTOLOG\u00cdA<\/h3>\n
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\nBlvd. Juriquilla 3001, Juriquilla
\nQuer\u00e9taro, M\u00e9xico, 76230
\nCd. de Quer\u00e9taro:238-1033
\nOtras Ciudades: (442)238-1033<\/p>\nMayores Informes<\/strong>
\nElsa Nydia Hernandez R\u00edos
\nhrios@unam.mx<\/p>\nDra. Ericka A. de los R\u00edos Arellano
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SOLICITUD DE SERVICIO
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SERVICIO DE HISTOLOG\u00cdA<\/h3>\n
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Como parte de los procesos de apoyo y fortalecimiento a la investigaci\u00f3n, la Unidad de Microscop\u00eda ofrece el servicio de t\u00e9cnicas histol\u00f3gicas para la visualizaci\u00f3n y an\u00e1lisis de tejidos en distintas condiciones fisiopatol\u00f3gicas.
Para lo cual la unidad cuenta con los siguientes servicios de \u00e1rea de histolog\u00eda:<\/p>\n
\n- Deshidrataci\u00f3n<\/strong><\/li>\n
- Inclusi\u00f3n<\/strong><\/li>\n
- Cortes de tejidos en Micr\u00f3tomo, Criostato y Vibratomo<\/strong><\/li>\n
- T\u00e9cnicas de tinci\u00f3n<\/strong><\/li>\n
- T\u00e9cnicas de inmunohistoqu\u00edmica e inmunofluorescencia<\/strong><\/li>\n
- Capacitaci\u00f3n y certificaci\u00f3n para el uso de equipos del \u00e1rea de histolog\u00eda<\/strong><\/li>\n
- Asesor\u00eda para el an\u00e1lisis cuantitativo de las secciones histol\u00f3gicas<\/strong><\/li>\n<\/ul>\n
Para solicitar los servicios del \u00e1rea de histolog\u00eda se pone a disposici\u00f3n de los usuarios el formato de Solicitud de servicio de Histolog\u00eda<\/strong>, formato para la Solicitud de Uso de Equipos de Histolog\u00eda<\/strong> y la Carta aval para Uso de Equipos de Histolog\u00eda.<\/strong><\/p>\n <\/p>\n
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PROCESOS DE HISTOLOG\u00cdA<\/b><\/p>\n
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USUARIOS SERVICIO DE HISTOLOG\u00cdA<\/b><\/p>\n
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REGLAMENTO DE LA UNIDAD<\/b><\/p>\n
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SOLICITUD DE SERVICIO DE HISTOLOG\u00cdA<\/b><\/p>\n
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CAT\u00c1LOGO DE HISTOLOG\u00cdA<\/b><\/p>\n
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VIDEOS DE HISTOLOG\u00cdA<\/b><\/p>\n
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PROCESOS DE HISTOLOG\u00cdA<\/b><\/p>\n
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USUARIOS SERVICIO DE HISTOLOG\u00cdA<\/b><\/p>\n
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REGLAMENTO DE LA UNIDAD<\/b><\/p>\n
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SOLICITUD DE SERVICIO DE HISTOLOG\u00cdA<\/b><\/p>\n
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CAT\u00c1LOGO DE HISTOLOG\u00cdA<\/b><\/p>\n
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VIDEOS DE HISTOLOG\u00cdA<\/b><\/p>\n
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UNIDAD DE PROTEOGEN\u00d3MICA<\/h2>\n
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Desarrollo de diversas t\u00e9cnicas de Biolog\u00eda Molecular y de cultivo celular, de acuerdo con las necesidades de los usuarios.<\/p>\n
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box_shadow_spread__hover_enabled=\u00bbon|hover\u00bb box_shadow_color__hover_enabled=\u00bbon|hover\u00bb box_shadow_horizontal__hover_enabled=\u00bbon|hover\u00bb box_shadow_vertical__hover_enabled=\u00bbon|desktop\u00bb]UNAM, CAMPUS JURIQUILLA<\/strong>
\nBlvd. Juriquilla 3001, Juriquilla
\nQuer\u00e9taro, M\u00e9xico, 76230
\nCd. de Quer\u00e9taro: 238-1034
\nOtras Ciudades: (442)238-1034<\/p>\n <\/p>\n
Responsable de la unidad<\/strong>
\nM. en C. Adriana Gonz\u00e1lez Gallardo
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Genotipificaci\u00f3n de Ratones<\/h3>\n
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PRODUCCI\u00d3N DE VIRUS<\/h3>\n
[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_text_color=\u00bb#425059″ text_font_size=\u00bb16px\u00bb text_line_height=\u00bb1em\u00bb header_font=\u00bb||||||||\u00bb header_3_font=\u00bb|||on|||||\u00bb header_3_text_color=\u00bb#ffffff\u00bb header_3_font_size=\u00bb18px\u00bb header_3_letter_spacing=\u00bb1px\u00bb header_3_line_height=\u00bb1.2em\u00bb background_color=\u00bb#8794A3″ background_layout=\u00bbdark\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbcenter\u00bb custom_margin=\u00bb1px|31px|18px|0px|false|false\u00bb custom_padding=\u00bb8px|10px|0px|10px|false|true\u00bb animation_style=\u00bbslide\u00bb animation_direction=\u00bbleft\u00bb animation_intensity_slide=\u00bb1%\u00bb link_option_url=\u00bb#varioskan\u00bb header_3_font_size_tablet=\u00bb\u00bb header_3_font_size_phone=\u00bb15px\u00bb header_3_font_size_last_edited=\u00bbon|desktop\u00bb box_shadow_style=\u00bbpreset2″ box_shadow_blur=\u00bb14px\u00bb locked=\u00bboff\u00bb text_line_height__hover=\u00bb1.7em\u00bb text_line_height__hover_enabled=\u00bb1.7em\u00bb box_shadow_blur__hover=\u00bb59px\u00bb box_shadow_blur__hover_enabled=\u00bbon|hover\u00bb background_layout__hover=\u00bblight\u00bb background_layout__hover_enabled=\u00bblight\u00bb text_font_size__hover=\u00bb14px\u00bb text_font_size__hover_enabled=\u00bb14px\u00bb text_letter_spacing__hover=\u00bb0px\u00bb text_letter_spacing__hover_enabled=\u00bb0px\u00bb text_text_shadow_style__hover=\u00bbnone\u00bb text_text_shadow_style__hover_enabled=\u00bbnone\u00bb text_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb text_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb link_font_size__hover=\u00bb14px\u00bb link_font_size__hover_enabled=\u00bb14px\u00bb link_letter_spacing__hover=\u00bb0px\u00bb link_letter_spacing__hover_enabled=\u00bb0px\u00bb link_line_height__hover=\u00bb1em\u00bb link_line_height__hover_enabled=\u00bb1em\u00bb link_text_shadow_style__hover=\u00bbnone\u00bb link_text_shadow_style__hover_enabled=\u00bbnone\u00bb link_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb link_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb ul_font_size__hover=\u00bb14px\u00bb ul_font_size__hover_enabled=\u00bb14px\u00bb ul_letter_spacing__hover=\u00bb0px\u00bb ul_letter_spacing__hover_enabled=\u00bb0px\u00bb ul_line_height__hover=\u00bb1em\u00bb ul_line_height__hover_enabled=\u00bb1em\u00bb ul_text_shadow_style__hover=\u00bbnone\u00bb ul_text_shadow_style__hover_enabled=\u00bbnone\u00bb ul_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb ul_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb ol_font_size__hover=\u00bb14px\u00bb ol_font_size__hover_enabled=\u00bb14px\u00bb ol_letter_spacing__hover=\u00bb0px\u00bb ol_letter_spacing__hover_enabled=\u00bb0px\u00bb ol_line_height__hover=\u00bb1em\u00bb ol_line_height__hover_enabled=\u00bb1em\u00bb ol_text_shadow_style__hover=\u00bbnone\u00bb ol_text_shadow_style__hover_enabled=\u00bbnone\u00bb ol_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb ol_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb quote_font_size__hover=\u00bb14px\u00bb quote_font_size__hover_enabled=\u00bb14px\u00bb quote_letter_spacing__hover=\u00bb0px\u00bb quote_letter_spacing__hover_enabled=\u00bb0px\u00bb quote_line_height__hover=\u00bb1em\u00bb quote_line_height__hover_enabled=\u00bb1em\u00bb quote_text_shadow_style__hover=\u00bbnone\u00bb quote_text_shadow_style__hover_enabled=\u00bbnone\u00bb quote_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb quote_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb header_5_font_size__hover=\u00bb16px\u00bb header_5_font_size__hover_enabled=\u00bb16px\u00bb header_5_letter_spacing__hover=\u00bb0px\u00bb header_5_letter_spacing__hover_enabled=\u00bb0px\u00bb header_5_line_height__hover=\u00bb1em\u00bb header_5_line_height__hover_enabled=\u00bb1em\u00bb header_5_text_shadow_style__hover=\u00bbnone\u00bb header_5_text_shadow_style__hover_enabled=\u00bbnone\u00bb header_5_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb header_5_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb text_orientation__hover=\u00bbleft\u00bb text_orientation__hover_enabled=\u00bbleft\u00bb background_enable_color__hover=\u00bbon\u00bb box_shadow_spread__hover_enabled=\u00bbon|hover\u00bb box_shadow_color__hover_enabled=\u00bbon|hover\u00bb box_shadow_color__hover=\u00bbrgba(0,0,0,0.38)\u00bb text_text_color__hover_enabled=\u00bbon|hover\u00bb text_text_color__hover=\u00bb#ffffff\u00bb header_3_font_size__hover_enabled=\u00bbon|hover\u00bb header_3_font_size__hover=\u00bb18px\u00bb background_color__hover=\u00bb#ffD400″ background__hover_enabled=\u00bbon|hover\u00bb]<\/p>\n
VARIOSKAN<\/h3>\n
[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_text_color=\u00bb#425059″ text_font_size=\u00bb16px\u00bb text_line_height=\u00bb1em\u00bb header_font=\u00bb||||||||\u00bb header_3_font=\u00bb|||on|||||\u00bb header_3_text_color=\u00bb#ffffff\u00bb header_3_font_size=\u00bb18px\u00bb header_3_letter_spacing=\u00bb1px\u00bb header_3_line_height=\u00bb1.2em\u00bb background_color=\u00bb#8794A3″ background_layout=\u00bbdark\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbcenter\u00bb custom_margin=\u00bb19px|31px|18px|0px|false|false\u00bb custom_padding=\u00bb8px|10px|0px|10px|false|true\u00bb animation_style=\u00bbslide\u00bb animation_direction=\u00bbleft\u00bb animation_intensity_slide=\u00bb1%\u00bb link_option_url=\u00bb#cdecultivo\u00bb header_3_font_size_tablet=\u00bb\u00bb header_3_font_size_phone=\u00bb15px\u00bb header_3_font_size_last_edited=\u00bbon|desktop\u00bb box_shadow_style=\u00bbpreset2″ box_shadow_blur=\u00bb14px\u00bb locked=\u00bboff\u00bb text_line_height__hover=\u00bb1.7em\u00bb text_line_height__hover_enabled=\u00bb1.7em\u00bb box_shadow_blur__hover=\u00bb59px\u00bb box_shadow_blur__hover_enabled=\u00bbon|hover\u00bb background_layout__hover=\u00bblight\u00bb background_layout__hover_enabled=\u00bblight\u00bb text_font_size__hover=\u00bb14px\u00bb text_font_size__hover_enabled=\u00bb14px\u00bb text_letter_spacing__hover=\u00bb0px\u00bb text_letter_spacing__hover_enabled=\u00bb0px\u00bb text_text_shadow_style__hover=\u00bbnone\u00bb text_text_shadow_style__hover_enabled=\u00bbnone\u00bb text_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb text_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb link_font_size__hover=\u00bb14px\u00bb link_font_size__hover_enabled=\u00bb14px\u00bb link_letter_spacing__hover=\u00bb0px\u00bb link_letter_spacing__hover_enabled=\u00bb0px\u00bb link_line_height__hover=\u00bb1em\u00bb link_line_height__hover_enabled=\u00bb1em\u00bb link_text_shadow_style__hover=\u00bbnone\u00bb link_text_shadow_style__hover_enabled=\u00bbnone\u00bb link_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb link_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb ul_font_size__hover=\u00bb14px\u00bb ul_font_size__hover_enabled=\u00bb14px\u00bb ul_letter_spacing__hover=\u00bb0px\u00bb ul_letter_spacing__hover_enabled=\u00bb0px\u00bb ul_line_height__hover=\u00bb1em\u00bb ul_line_height__hover_enabled=\u00bb1em\u00bb ul_text_shadow_style__hover=\u00bbnone\u00bb ul_text_shadow_style__hover_enabled=\u00bbnone\u00bb ul_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb ul_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb ol_font_size__hover=\u00bb14px\u00bb ol_font_size__hover_enabled=\u00bb14px\u00bb ol_letter_spacing__hover=\u00bb0px\u00bb ol_letter_spacing__hover_enabled=\u00bb0px\u00bb ol_line_height__hover=\u00bb1em\u00bb ol_line_height__hover_enabled=\u00bb1em\u00bb ol_text_shadow_style__hover=\u00bbnone\u00bb ol_text_shadow_style__hover_enabled=\u00bbnone\u00bb ol_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb ol_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb quote_font_size__hover=\u00bb14px\u00bb quote_font_size__hover_enabled=\u00bb14px\u00bb quote_letter_spacing__hover=\u00bb0px\u00bb quote_letter_spacing__hover_enabled=\u00bb0px\u00bb quote_line_height__hover=\u00bb1em\u00bb quote_line_height__hover_enabled=\u00bb1em\u00bb quote_text_shadow_style__hover=\u00bbnone\u00bb quote_text_shadow_style__hover_enabled=\u00bbnone\u00bb quote_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb quote_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb header_5_font_size__hover=\u00bb16px\u00bb header_5_font_size__hover_enabled=\u00bb16px\u00bb header_5_letter_spacing__hover=\u00bb0px\u00bb header_5_letter_spacing__hover_enabled=\u00bb0px\u00bb header_5_line_height__hover=\u00bb1em\u00bb header_5_line_height__hover_enabled=\u00bb1em\u00bb header_5_text_shadow_style__hover=\u00bbnone\u00bb header_5_text_shadow_style__hover_enabled=\u00bbnone\u00bb header_5_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb header_5_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb text_orientation__hover=\u00bbleft\u00bb text_orientation__hover_enabled=\u00bbleft\u00bb background__hover_enabled=\u00bbon|hover\u00bb background_color__hover=\u00bb#ffa23f\u00bb background_enable_color__hover=\u00bbon\u00bb box_shadow_spread__hover_enabled=\u00bbon|hover\u00bb box_shadow_color__hover_enabled=\u00bbon|hover\u00bb box_shadow_color__hover=\u00bbrgba(0,0,0,0.38)\u00bb text_text_color__hover_enabled=\u00bbon|hover\u00bb text_text_color__hover=\u00bb#ffffff\u00bb header_3_font_size__hover_enabled=\u00bbon|hover\u00bb header_3_font_size__hover=\u00bb18px\u00bb]<\/p>\n
CUARTO DE CULTIVO<\/h3>\n
[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_text_color=\u00bb#425059″ text_font_size=\u00bb16px\u00bb text_line_height=\u00bb1em\u00bb header_font=\u00bb||||||||\u00bb header_3_font=\u00bb|||on|||||\u00bb header_3_text_color=\u00bb#ffffff\u00bb header_3_font_size=\u00bb18px\u00bb header_3_letter_spacing=\u00bb1px\u00bb header_3_line_height=\u00bb1.2em\u00bb background_color=\u00bb#8794A3″ background_layout=\u00bbdark\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbcenter\u00bb custom_margin=\u00bb1px|31px|18px|0px|false|false\u00bb custom_padding=\u00bb8px|10px|0px|10px|false|true\u00bb animation_style=\u00bbslide\u00bb animation_direction=\u00bbleft\u00bb animation_intensity_slide=\u00bb1%\u00bb link_option_url=\u00bb#pcr\u00bb header_3_font_size_tablet=\u00bb\u00bb header_3_font_size_phone=\u00bb15px\u00bb header_3_font_size_last_edited=\u00bbon|phone\u00bb box_shadow_style=\u00bbpreset2″ box_shadow_blur=\u00bb14px\u00bb locked=\u00bboff\u00bb text_line_height__hover=\u00bb1.7em\u00bb text_line_height__hover_enabled=\u00bb1.7em\u00bb box_shadow_blur__hover=\u00bb59px\u00bb box_shadow_blur__hover_enabled=\u00bbon|hover\u00bb background_layout__hover=\u00bblight\u00bb background_layout__hover_enabled=\u00bblight\u00bb text_font_size__hover=\u00bb14px\u00bb text_font_size__hover_enabled=\u00bb14px\u00bb text_letter_spacing__hover=\u00bb0px\u00bb text_letter_spacing__hover_enabled=\u00bb0px\u00bb text_text_shadow_style__hover=\u00bbnone\u00bb text_text_shadow_style__hover_enabled=\u00bbnone\u00bb text_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb text_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb link_font_size__hover=\u00bb14px\u00bb link_font_size__hover_enabled=\u00bb14px\u00bb link_letter_spacing__hover=\u00bb0px\u00bb link_letter_spacing__hover_enabled=\u00bb0px\u00bb link_line_height__hover=\u00bb1em\u00bb link_line_height__hover_enabled=\u00bb1em\u00bb link_text_shadow_style__hover=\u00bbnone\u00bb link_text_shadow_style__hover_enabled=\u00bbnone\u00bb link_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb link_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb ul_font_size__hover=\u00bb14px\u00bb ul_font_size__hover_enabled=\u00bb14px\u00bb ul_letter_spacing__hover=\u00bb0px\u00bb ul_letter_spacing__hover_enabled=\u00bb0px\u00bb ul_line_height__hover=\u00bb1em\u00bb ul_line_height__hover_enabled=\u00bb1em\u00bb ul_text_shadow_style__hover=\u00bbnone\u00bb ul_text_shadow_style__hover_enabled=\u00bbnone\u00bb ul_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb ul_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb ol_font_size__hover=\u00bb14px\u00bb ol_font_size__hover_enabled=\u00bb14px\u00bb ol_letter_spacing__hover=\u00bb0px\u00bb ol_letter_spacing__hover_enabled=\u00bb0px\u00bb ol_line_height__hover=\u00bb1em\u00bb ol_line_height__hover_enabled=\u00bb1em\u00bb ol_text_shadow_style__hover=\u00bbnone\u00bb ol_text_shadow_style__hover_enabled=\u00bbnone\u00bb ol_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb ol_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb quote_font_size__hover=\u00bb14px\u00bb quote_font_size__hover_enabled=\u00bb14px\u00bb quote_letter_spacing__hover=\u00bb0px\u00bb quote_letter_spacing__hover_enabled=\u00bb0px\u00bb quote_line_height__hover=\u00bb1em\u00bb quote_line_height__hover_enabled=\u00bb1em\u00bb quote_text_shadow_style__hover=\u00bbnone\u00bb quote_text_shadow_style__hover_enabled=\u00bbnone\u00bb quote_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb quote_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb header_5_font_size__hover=\u00bb16px\u00bb header_5_font_size__hover_enabled=\u00bb16px\u00bb header_5_letter_spacing__hover=\u00bb0px\u00bb header_5_letter_spacing__hover_enabled=\u00bb0px\u00bb header_5_line_height__hover=\u00bb1em\u00bb header_5_line_height__hover_enabled=\u00bb1em\u00bb header_5_text_shadow_style__hover=\u00bbnone\u00bb header_5_text_shadow_style__hover_enabled=\u00bbnone\u00bb header_5_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb header_5_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb text_orientation__hover=\u00bbleft\u00bb text_orientation__hover_enabled=\u00bbleft\u00bb background_enable_color__hover=\u00bbon\u00bb box_shadow_spread__hover_enabled=\u00bbon|hover\u00bb box_shadow_color__hover_enabled=\u00bbon|hover\u00bb box_shadow_color__hover=\u00bbrgba(0,0,0,0.38)\u00bb text_text_color__hover_enabled=\u00bbon|hover\u00bb text_text_color__hover=\u00bb#ffffff\u00bb header_3_font_size__hover_enabled=\u00bbon|desktop\u00bb header_3_font_size__hover=\u00bb18px\u00bb background_color__hover=\u00bb#ffD400″ background__hover_enabled=\u00bbon|hover\u00bb]<\/p>\n
PCR<\/h3>\n
[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_text_color=\u00bb#425059″ text_font_size=\u00bb16px\u00bb text_line_height=\u00bb1em\u00bb header_font=\u00bb||||||||\u00bb header_3_font=\u00bb|||on|||||\u00bb header_3_text_color=\u00bb#ffffff\u00bb header_3_font_size=\u00bb18px\u00bb header_3_letter_spacing=\u00bb1px\u00bb header_3_line_height=\u00bb1.2em\u00bb background_color=\u00bb#8794A3″ background_layout=\u00bbdark\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbcenter\u00bb custom_margin=\u00bb1px|31px|18px|0px|false|false\u00bb custom_padding=\u00bb8px|10px|0px|10px|false|true\u00bb animation_style=\u00bbslide\u00bb animation_direction=\u00bbleft\u00bb animation_intensity_slide=\u00bb1%\u00bb link_option_url=\u00bb#pcrentreal\u00bb header_3_font_size_tablet=\u00bb\u00bb header_3_font_size_phone=\u00bb15px\u00bb header_3_font_size_last_edited=\u00bbon|desktop\u00bb box_shadow_style=\u00bbpreset2″ box_shadow_blur=\u00bb14px\u00bb locked=\u00bboff\u00bb text_line_height__hover=\u00bb1.7em\u00bb text_line_height__hover_enabled=\u00bb1.7em\u00bb box_shadow_blur__hover=\u00bb59px\u00bb box_shadow_blur__hover_enabled=\u00bbon|hover\u00bb background_layout__hover=\u00bblight\u00bb background_layout__hover_enabled=\u00bblight\u00bb text_font_size__hover=\u00bb14px\u00bb text_font_size__hover_enabled=\u00bb14px\u00bb text_letter_spacing__hover=\u00bb0px\u00bb text_letter_spacing__hover_enabled=\u00bb0px\u00bb text_text_shadow_style__hover=\u00bbnone\u00bb text_text_shadow_style__hover_enabled=\u00bbnone\u00bb text_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb text_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb link_font_size__hover=\u00bb14px\u00bb link_font_size__hover_enabled=\u00bb14px\u00bb link_letter_spacing__hover=\u00bb0px\u00bb link_letter_spacing__hover_enabled=\u00bb0px\u00bb link_line_height__hover=\u00bb1em\u00bb link_line_height__hover_enabled=\u00bb1em\u00bb link_text_shadow_style__hover=\u00bbnone\u00bb link_text_shadow_style__hover_enabled=\u00bbnone\u00bb link_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb link_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb ul_font_size__hover=\u00bb14px\u00bb ul_font_size__hover_enabled=\u00bb14px\u00bb ul_letter_spacing__hover=\u00bb0px\u00bb ul_letter_spacing__hover_enabled=\u00bb0px\u00bb ul_line_height__hover=\u00bb1em\u00bb ul_line_height__hover_enabled=\u00bb1em\u00bb ul_text_shadow_style__hover=\u00bbnone\u00bb ul_text_shadow_style__hover_enabled=\u00bbnone\u00bb ul_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb ul_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb ol_font_size__hover=\u00bb14px\u00bb ol_font_size__hover_enabled=\u00bb14px\u00bb ol_letter_spacing__hover=\u00bb0px\u00bb ol_letter_spacing__hover_enabled=\u00bb0px\u00bb ol_line_height__hover=\u00bb1em\u00bb ol_line_height__hover_enabled=\u00bb1em\u00bb ol_text_shadow_style__hover=\u00bbnone\u00bb ol_text_shadow_style__hover_enabled=\u00bbnone\u00bb ol_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb ol_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb quote_font_size__hover=\u00bb14px\u00bb quote_font_size__hover_enabled=\u00bb14px\u00bb quote_letter_spacing__hover=\u00bb0px\u00bb quote_letter_spacing__hover_enabled=\u00bb0px\u00bb quote_line_height__hover=\u00bb1em\u00bb quote_line_height__hover_enabled=\u00bb1em\u00bb quote_text_shadow_style__hover=\u00bbnone\u00bb quote_text_shadow_style__hover_enabled=\u00bbnone\u00bb quote_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb quote_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb header_5_font_size__hover=\u00bb16px\u00bb header_5_font_size__hover_enabled=\u00bb16px\u00bb header_5_letter_spacing__hover=\u00bb0px\u00bb header_5_letter_spacing__hover_enabled=\u00bb0px\u00bb header_5_line_height__hover=\u00bb1em\u00bb header_5_line_height__hover_enabled=\u00bb1em\u00bb header_5_text_shadow_style__hover=\u00bbnone\u00bb header_5_text_shadow_style__hover_enabled=\u00bbnone\u00bb header_5_text_shadow_color__hover=\u00bbrgba(0,0,0,0.4)\u00bb header_5_text_shadow_color__hover_enabled=\u00bbrgba(0,0,0,0.4)\u00bb text_orientation__hover=\u00bbleft\u00bb text_orientation__hover_enabled=\u00bbleft\u00bb background_enable_color__hover=\u00bbon\u00bb box_shadow_spread__hover_enabled=\u00bbon|hover\u00bb box_shadow_color__hover_enabled=\u00bbon|hover\u00bb box_shadow_color__hover=\u00bbrgba(0,0,0,0.38)\u00bb text_text_color__hover_enabled=\u00bbon|hover\u00bb text_text_color__hover=\u00bb#ffffff\u00bb header_3_font_size__hover_enabled=\u00bbon|hover\u00bb header_3_font_size__hover=\u00bb18px\u00bb background_color__hover=\u00bb#ffD400″ background__hover_enabled=\u00bbon|hover\u00bb]<\/p>\n
PCR EN TIEMPO REAL<\/h3>\n
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CLONACI\u00d3N DE PRODUCTOD PCR<\/h3>\n
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PREPARACI\u00d3N DE PL\u00c1SMIDOS MAXI \/ C\u00c9LULAS COMPETENTES \/ MEDIOS<\/h3>\n
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FOTODOCUMENTACI\u00d3N \/ STORM<\/h3>\n
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CURSOS DISPONIBLES<\/h3>\n
[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_font_size=\u00bb12px\u00bb text_line_height=\u00bb1.4em\u00bb background_color=\u00bb#ffffff\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbleft\u00bb custom_margin=\u00bb72px|0px|13px|0px|false|false\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb25px||||false|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb custom_padding=\u00bb4px|7px|4px|10px|true|false\u00bb custom_padding_last_edited=\u00bboff|desktop\u00bb link_option_url=\u00bbhttp:\/\/132.248.142.6:5000\/fsdownload\/fbsharing-OXadZ3eX\/Manuales\u00bb link_option_url_new_window=\u00bbon\u00bb border_width_all=\u00bb1px\u00bb border_color_all=\u00bb#aebac1″ border_width_right=\u00bb0px\u00bb border_width_left=\u00bb6px\u00bb border_color_left=\u00bb#425059″ box_shadow_style=\u00bbpreset1″ box_shadow_vertical=\u00bb0px\u00bb box_shadow_blur=\u00bb0px\u00bb locked=\u00bboff\u00bb border_width_left__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon\u00bb custom_padding__hover_enabled=\u00bbon\u00bb custom_padding__hover=\u00bb|32px||\u00bb custom_margin__hover=\u00bb|||-8px\u00bb box_shadow_blur__hover=\u00bb40px\u00bb border_color_left__hover_enabled=\u00bbon|hover\u00bb border_color_left__hover=\u00bb#FF6600″ border_width_left__hover=\u00bb8px\u00bb]<\/p>\n
REGLAMENTO INTERNO DE LA UNIDAD DE PROTEOGEN\u00d3MICA<\/strong><\/p>\n[\/et_pb_text][et_pb_button button_url=\u00bb#unidadesdeapoyo\u00bb button_text=\u00bbUNIDADES DE APOYO A LA INVESTIGACION\u00bb button_alignment=\u00bbleft\u00bb _builder_version=\u00bb4.4.0″ custom_button=\u00bbon\u00bb button_text_size=\u00bb15px\u00bb button_text_color=\u00bb#ffffff\u00bb button_bg_color=\u00bb#8794A3″ button_border_width=\u00bb0px\u00bb button_border_color=\u00bbrgba(0,0,0,0)\u00bb button_border_radius=\u00bb0px\u00bb button_letter_spacing=\u00bb1px\u00bb button_font=\u00bbOswald||||||||\u00bb button_icon=\u00bb%%179%%\u00bb button_icon_color=\u00bb#ffffff\u00bb button_icon_placement=\u00bbleft\u00bb button_on_hover=\u00bboff\u00bb custom_margin=\u00bb25px||38px|||\u00bb button_text_size_tablet=\u00bb\u00bb button_text_size_phone=\u00bb12px\u00bb button_text_size_last_edited=\u00bbon|phone\u00bb locked=\u00bboff\u00bb button_bg_color__hover_enabled=\u00bbon|hover\u00bb 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INFORMACI\u00d3N GENERAL<\/h3>\n
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La Unidad de Proteogen\u00f3mica cuenta con el equipamiento y material necesario para mantener y transfectar c\u00e9lulas eucariontes, para preparar c\u00e9lulas competentes, para preparar medios de cultivo para bacterias, entre otras t\u00e9cnicas moleculares, de acuerdo con las necesidades de los usuarios. Cuenta con diversos equipos muy especializados (STORM, Varioskan, LightCycler, ultracentrifuga Beckman, entre otros). La Unidad contiene un cuarto frio y espacios protegidos para trabajar con cultivos celulares y radioactividad.<\/p>\n
La Unidad ofrece sus servicios tanto a usuarios internos como externos, brinda asesor\u00edas y materiales especializados en m\u00e9todos bioqu\u00edmicos y moleculares, y cuenta con un programa peri\u00f3dico de cursos te\u00f3ricos-pr\u00e1cticos de biolog\u00eda molecular, PCR en tiempo real y bioinform\u00e1tica.<\/p>\n
Adem\u00e1s, la Unidad ofrece su apoyo a los usuarios internos y externos en el an\u00e1lisis bioinform\u00e1tico. Tenemos experiencia en ensamble de transcriptomas, expresi\u00f3n diferencial de genes, an\u00e1lisis de indels e identificaci\u00f3n de prote\u00ednas novedosas y estamos dispuestos a expandir a otras t\u00e9cnicas de acuerdo a los requerimientos de los colaboradores. Los flujos de trabajo est\u00e1n dise\u00f1ados en base a las necesidades de los usuarios y est\u00e1n disponibles para su uso en publicaciones cuando estos son requeridos por los revisores. Este servicio se ofrece sin costo a los investigadores y alumnos del INB.<\/p>\n
De los trabajos realizados exitosamente en la Unidad se debe de dar el agradecimiento en los manuscritos publicados, res\u00famenes en congresos o presentaciones de la contribuci\u00f3n de la M. en C. Adriana Gonz\u00e1lez Gallardo y\/o el Dr. Michael C. Jeziorski y\/o\u00a0Dra. Anaid Antarami\u00e1n Salas, seg\u00fan sea el caso.<\/p>\n
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SECUENCIA DE \u00c1CIDOS NUCLEICOS<\/h3>\n
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UNAM, Campus Juriquilla<\/strong>
Blvd. Juriquilla 3001, Juriquilla
Quer\u00e9taro, M\u00e9xico, 76230
Cd. de Quer\u00e9taro:238-1033
Otras Ciudades: (442)238-1033<\/p>\nMayores Informes<\/strong>
Dr. Michael Jeziorski
jeziorsk@unam.mx<\/p>\n[\/et_pb_text][et_pb_text _builder_version=\u00bb4.4.0″ text_font=\u00bb|600|||||||\u00bb text_font_size=\u00bb12px\u00bb text_line_height=\u00bb1.4em\u00bb background_color=\u00bb#ffffff\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbleft\u00bb custom_margin=\u00bb72px|0px|13px|0px|false|false\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb25px|0px|13px|0px|false|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb custom_padding=\u00bb4px|7px|4px|10px|true|false\u00bb custom_padding_last_edited=\u00bboff|desktop\u00bb link_option_url=\u00bbhttp:\/\/132.248.142.11\/upg_seq\/sequence_requests\/new\u00bb link_option_url_new_window=\u00bbon\u00bb border_width_all=\u00bb1px\u00bb border_color_all=\u00bb#aebac1″ border_width_right=\u00bb0px\u00bb border_width_left=\u00bb6px\u00bb border_color_left=\u00bb#425059″ box_shadow_style=\u00bbpreset1″ box_shadow_vertical=\u00bb0px\u00bb box_shadow_blur=\u00bb0px\u00bb locked=\u00bboff\u00bb border_width_left__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon\u00bb custom_padding__hover_enabled=\u00bbon\u00bb custom_padding__hover=\u00bb|32px||\u00bb custom_margin__hover=\u00bb|||-8px\u00bb box_shadow_blur__hover=\u00bb40px\u00bb border_color_left__hover_enabled=\u00bbon|hover\u00bb border_color_left__hover=\u00bb#FF6600″ border_width_left__hover=\u00bb8px\u00bb]<\/p>\n
SOLICITUD DE SECUENCIAS DE ACIDOS NUCLEICOS<\/strong><\/p>\n[\/et_pb_text][et_pb_text _builder_version=\u00bb3.27.4″ text_font=\u00bb|600|||||||\u00bb text_font_size=\u00bb12px\u00bb text_line_height=\u00bb1.2em\u00bb background_color=\u00bb#ffffff\u00bb width=\u00bb100%\u00bb module_alignment=\u00bbleft\u00bb custom_margin=\u00bb20px|0px|13px|0px|false|false\u00bb custom_margin_last_edited=\u00bboff|desktop\u00bb custom_padding=\u00bb6px|7px|6px|10px|true|false\u00bb custom_padding_last_edited=\u00bboff|desktop\u00bb link_option_url=\u00bbhttp:\/\/www.inb.unam.mx\/wp-content\/uploads\/2019\/11\/rec_servicio_an.pdf\u00bb link_option_url_new_window=\u00bbon\u00bb border_width_all=\u00bb1px\u00bb border_color_all=\u00bb#aebac1″ border_width_right=\u00bb0px\u00bb border_width_left=\u00bb6px\u00bb border_color_left=\u00bb#425059″ box_shadow_style=\u00bbpreset1″ box_shadow_vertical=\u00bb0px\u00bb box_shadow_blur=\u00bb0px\u00bb locked=\u00bboff\u00bb border_width_left__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon\u00bb custom_padding__hover_enabled=\u00bbon\u00bb custom_padding__hover=\u00bb|32px||\u00bb custom_margin__hover=\u00bb|||-8px\u00bb box_shadow_blur__hover=\u00bb40px\u00bb border_color_left__hover_enabled=\u00bbon|hover\u00bb border_color_left__hover=\u00bb#FF6600″ border_width_left__hover=\u00bb8px\u00bb]<\/p>\n
RECOMENDACIONES DE SERVICIO<\/strong><\/p>\n[\/et_pb_text][et_pb_button button_url=\u00bb#proteogenomica\u00bb button_text=\u00bbUNIDAD DE PROTEOGEN\u00d3MICA\u00bb button_alignment=\u00bbleft\u00bb _builder_version=\u00bb4.4.0″ custom_button=\u00bbon\u00bb button_text_size=\u00bb15px\u00bb button_text_color=\u00bb#ffffff\u00bb button_bg_color=\u00bb#8794A3″ button_border_width=\u00bb0px\u00bb button_border_color=\u00bbrgba(0,0,0,0)\u00bb button_border_radius=\u00bb0px\u00bb button_letter_spacing=\u00bb1px\u00bb button_font=\u00bbOswald||||||||\u00bb button_icon=\u00bb%%179%%\u00bb button_icon_color=\u00bb#ffffff\u00bb button_icon_placement=\u00bbleft\u00bb button_on_hover=\u00bboff\u00bb custom_margin=\u00bb25px|||||\u00bb button_text_size_tablet=\u00bb\u00bb button_text_size_phone=\u00bb12px\u00bb button_text_size_last_edited=\u00bbon|phone\u00bb locked=\u00bboff\u00bb button_bg_color__hover_enabled=\u00bbon|hover\u00bb button_bg_color__hover=\u00bb#ffD400″ 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SECUENCIA DE \u00c1CIDOS NUCLEICOS<\/h3>\n
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Nuevos requisitos para el servicio de secuenciaci\u00f3n Sanger:<\/strong><\/h1>\nEl solicitante debe entregar las muestras y los oligos en la Unidad de Proteogen\u00f3mica (B-01).<\/p>\n
Cada reacci\u00f3n consiste de una muestra de ADN (pl\u00e1smido o PCR) y un oligo.<\/p>\n
Los requisitos que deben cumplir los pl\u00e1smidos son:<\/p>\n
\n- \n
Purificados y cuantificados<\/p>\n<\/li>\n
- \n
Concentraci\u00f3n ajustada entre 150\u2013200 ng\/\u00b5l<\/strong><\/p>\n<\/li>\n- \n
Se requieren 10\u201315 \u00b5l m\u00e1s<\/strong> para reacciones m\u00faltiples<\/p>\n<\/li>\n<\/ul>\nLos requisitos que deben cumplir los productos de PCR son:<\/p>\n
\n- \n
Purificados y cuantificados<\/p>\n<\/li>\n
- \n
Concentraci\u00f3n ajustada a 50 ng\/\u00b5l (no puede ser menor)<\/strong><\/p>\n<\/li>\n- \n
Se requieren 10\u201315 \u00b5l m\u00e1s<\/strong> para reacciones m\u00faltiples<\/p>\n<\/li>\n- \n
Una foto del gel de agarosa del producto analizado a una concentraci\u00f3n de 50 ng\/\u00b5l<\/strong>, indicando el volumen de carga y el tama\u00f1o del producto<\/p>\n<\/li>\n<\/ul>\nLos requisitos para los oligos son:<\/p>\n
\n- \n
10 \u00b5M = 10 pmol\/\u00b5l<\/strong><\/p>\n<\/li>\n- \n
5 \u00b5l<\/strong> del oligo para cada reacci\u00f3n solicitada<\/p>\n<\/li>\n- \n
No es necesario entregar diluciones de los oligos M13 forward y reverse, T7, SP6 o T3<\/strong><\/p>\n<\/li>\n<\/ul>\nLas muestras y los oligos se colocan en la gradilla amarilla que se encuentra en la tarja del cuarto fr\u00edo de la Unidad. Los tubos deben ser de 0.5 o 1.5 ml (no 0.2 ml)<\/strong>, identificados con nombres legibles y sus concentraciones.<\/p>\nAdem\u00e1s, el solicitante debe llenar un formato de solicitud del servicio con su nombre, jefe(a) del grupo, laboratorio y correo electr\u00f3nico. Se escribe cada reacci\u00f3n solicitada en una l\u00ednea separada con los siguientes datos: nombre de la muestra, tipo de muestra (pl\u00e1smido o PCR), nombre del oligo. Los nombres tienen que ser claros, cortos, \u00fanicos y deben corresponder exactamente a las identificaciones de los tubos. Si hay discrepancia en los nombres entre la solicitud y los tubos, no se env\u00edan las muestras.<\/p>\n
Las muestras se env\u00edan los mi\u00e9rcoles por la tarde<\/strong> al Laboratorio de Biolog\u00eda Molecular del Instituto de Fisiolog\u00eda Celular, Ciudad Universitaria. En caso de que un solicitante no pueda entregar sus muestras el mi\u00e9rcoles y requiera m\u00e1s tiempo, ser\u00e1 posible demorar el env\u00edo de las muestras siempre y cuando avise con 24 horas de anticipaci\u00f3n (el martes)<\/strong>. Si el env\u00edo est\u00e1 programado por valija universitaria<\/strong>, no ser\u00e1 posible la demora.<\/p>\nDebido a que el IFC no otorga cr\u00e9dito, los usuarios deber\u00e1n realizar el pago por adelantado del servicio a la Unidad de Proteogen\u00f3mica. Cada reacci\u00f3n tiene un costo de $250.00 MXN<\/strong>.<\/p>\n
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GENOTIPIFICACI\u00d3N DE RATONES<\/h3>\n
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UNAM, Campus Juriquilla<\/strong>
Blvd. Juriquilla 3001, Juriquilla
Quer\u00e9taro, M\u00e9xico, 76230
Cd. de Quer\u00e9taro:238-1033
Otras Ciudades: (442)238-1033<\/p>\nMayores Informes<\/strong>
M. en C. Adriana Gonz\u00e1lez Gallardo
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PROTOCOLO DE GENOTIPIFICACI\u00d3N<\/strong><\/p>\n[\/et_pb_text][et_pb_button button_url=\u00bb#proteogenomica\u00bb button_text=\u00bbUNIDAD DE PROTEOGEN\u00d3MICA\u00bb button_alignment=\u00bbleft\u00bb _builder_version=\u00bb4.4.0″ custom_button=\u00bbon\u00bb button_text_size=\u00bb15px\u00bb button_text_color=\u00bb#ffffff\u00bb button_bg_color=\u00bb#8794A3″ button_border_width=\u00bb0px\u00bb button_border_color=\u00bbrgba(0,0,0,0)\u00bb button_border_radius=\u00bb0px\u00bb button_letter_spacing=\u00bb1px\u00bb button_font=\u00bbOswald||||||||\u00bb button_icon=\u00bb%%179%%\u00bb button_icon_color=\u00bb#ffffff\u00bb button_icon_placement=\u00bbleft\u00bb button_on_hover=\u00bboff\u00bb custom_margin=\u00bb25px|||||\u00bb button_text_size_tablet=\u00bb\u00bb button_text_size_phone=\u00bb12px\u00bb button_text_size_last_edited=\u00bbon|phone\u00bb locked=\u00bboff\u00bb button_bg_color__hover_enabled=\u00bbon|hover\u00bb button_bg_color__hover=\u00bb#ffD400″ 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GENOTIPIFICACI\u00d3N DE RATONES<\/h3>\n
[\/et_pb_text][et_pb_text content_tablet=\u00bb\u00bb content_phone=\u00bbEn la Unidad de Proteogen\u00f3mica se ofrece el servicio de Genotipificaciones de ratones transg\u00e9nicos.
\nConsulte aqu\u00ed el Protocolo de Genotipificaci\u00f3n.<\/p>\n
Costo del Servicio:<\/strong> 50.00 pesos M.N. por muestra<\/p>\nRequisitos para una nueva cepa diferente a las que se tienen estandarizadas:<\/p>\n
1-2 mm de cola de rat\u00f3n
\n\u2022 50 microlitros de cada Oligonucel\u00f3tidos para llevar a cabo el PCR (10mM)
\n\u2022 Protocolo de PCR.<\/p>\n
CEPAS<\/strong><\/p>\n\n\n\n| Rat\u00f3n <\/span><\/td>\n | Kiss1 <\/span>tm1.1 (cre\/EGFP)stei\/<\/span>j<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | B6. Cg-Tg(APPSwFILon.PSEN1*M146L<\/span><\/p>\n *L286V)6799Vas\/Mmjax<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n <\/span><\/td>\n | B6. FVB(Cg)-Tg(Drd2-cre) ER44Gsat\/mmcd<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | B6.Cg-Tg(Drd1-cre)262Gsat\/Mmcd<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | Slc17a6<\/span>tm2(cre)Lowl\/<\/span>J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | B6J. 129S6(FVB)-SIc32a1<\/span>tm2(cre)LowI<\/span>\/MwarJ<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n <\/span><\/td>\n | B6.SJL-Sic6a3<\/span>tm1.1(cre)Bkmn<\/span>\/J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n <\/span><\/td>\n | C57BL\/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim\/J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R\/EYFP)Hze\/J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | B6N; 129 Tg(CAG-CHRM3*,-mcitrine)1Ute\/J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n <\/span><\/td>\n | Tg(Hcn4-GCaMP8)(B10-Mik\/J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | B6N.129-Gt(Rosa)26Sor<\/span>tm1(CAG-CHRM4*,-mCitrine)Ute<\/span>\/J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | B6J.B6N(Cg)Cx3Cr1<\/span>tm1.1(cre)Jung<\/span> \/J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | Tg(Cmk2a-tTA)1Mmay Fgf14 <\/span>Tg(tetO-MAPT*P301L)4510Kha<\/span>\/J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n <\/span><\/td>\n | B6.Cg-Tg(GFAP-cre\/eRT2)505Fmv\/J<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | PRKO<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | Lhx5 <\/span><\/td>\n<\/tr>\n\n| Rata<\/span><\/td>\n | RRRC 773 Pvalb-iCre<\/span><\/td>\n<\/tr>\n\n| Rata<\/span><\/td>\n | RRRC 767 Drd1a-iCre<\/span><\/td>\n<\/tr>\n\n| Rata<\/span><\/td>\n | RRRC 768 Drd2-iCre<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u00bb content_last_edited=\u00bbon|phone\u00bb _builder_version=\u00bb4.4.0″ text_font=\u00bb||||||||\u00bb text_text_color=\u00bb#425059″ text_font_size=\u00bb16px\u00bb text_line_height=\u00bb1.3em\u00bb header_font=\u00bb||||||||\u00bb header_3_font=\u00bbOswald|||on|||||\u00bb header_3_font_size=\u00bb26px\u00bb 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text_orientation__hover_enabled=\u00bbleft\u00bb background_layout__hover=\u00bblight\u00bb background_layout__hover_enabled=\u00bblight\u00bb border_radii__hover_enabled=\u00bbon|desktop\u00bb border_width_all__hover_enabled=\u00bbon|hover\u00bb border_color_all__hover_enabled=\u00bbon|hover\u00bb box_shadow_horizontal__hover_enabled=\u00bbon|hover\u00bb box_shadow_vertical__hover_enabled=\u00bbon|hover\u00bb box_shadow_blur__hover_enabled=\u00bbon|desktop\u00bb box_shadow_spread__hover_enabled=\u00bbon|hover\u00bb]<\/p>\n En la Unidad de Proteogen\u00f3mica se ofrece el servicio de Genotipificaciones de ratones transg\u00e9nicos.
Consulte aqu\u00ed el Protocolo de Genotipificaci\u00f3n.<\/p>\n Costo del Servicio:<\/strong>\u00a050.00 pesos M.N. por muestra<\/p>\nRequisitos para una nueva cepa diferente a las que se tienen estandarizadas:<\/p>\n 1-2 mm de cola de rat\u00f3n
\u2022\u00a050 microlitros de cada Oligonucel\u00f3tidos para llevar a cabo el PCR (10mM)
\u2022\u00a0Protocolo de PCR.<\/p>\n CEPAS<\/strong><\/p>\n\n\n\n| Rat\u00f3n\u00a0<\/span><\/td>\n | Kiss1 <\/span>tm1.1 (cre\/EGFP)stei\/<\/span>j<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | B6. Cg-Tg(APPSwFILon.PSEN1*M146L*L286V)6799Vas\/Mmjax<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n\u00a0<\/span><\/td>\n | B6. FVB(Cg)-Tg(Drd2-cre) ER44Gsat\/mmcd<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | B6.Cg-Tg(Drd1-cre)262Gsat\/Mmcd<\/span><\/td>\n<\/tr>\n\n| Rat\u00f3n<\/span><\/td>\n | Slc17a6<\/span>tm2(cre)Lowl\/<\/span>J<\/span><\/td>\n<\/tr>\n\n | | | | | | | | | | | | | | | | | | | | | | | | |